Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Research on Microbes
Microbiology Experts
Online Submission
Access Statistics

Site Visitors

blog tracking

Lecture Notes in Electrical Engineering
Vol. 250, 2014, Pages: 1041-1049

Isolation and Characterization of s-Butanol Tolerant Microorganisms

Litao Shi, Hongjiang Yang, Qian Li, Xuying Qin



s-butanol can be produced indirectly from biomass by fermentation. To construct recombinant strains producing s-butanol directly, microbes naturally with high tolerance to s-butanol are required for metabolic engineering. In this study, totally 30 s-butanol tolerant microorganisms were isolated from different environmental soil samples by screening and enrichment method. Among the isolates, strains S-2, S-9, S-24, and S-26 were able to tolerate 15 g/L s-butanol. Additionally, s-butanol tolerance mechanisms were also studied by quantitatively analyzing degradation ability of the isolates. The results indicated that strains S-9 and S-24 possessed relatively low levels of s-butanol degrading enzyme activity and might be used as native tolerant strains for metabolic engineering. Strain S-13 showed the highest s-butanol degradation efficiency (72.7 %) in LB medium, suggesting that strain S-13 disrupted s-butanol via a way of co-metabolism and suitable for bioremediation of the chemical under more complicated environments. Furthermore, the isolated strains were identified by comparative analysis of the 16S rRNA gene sequences.


Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution