Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Research on Microbes
Microbiology Experts
Online Submission

Site Visitors

blog tracking

Infection, Genetics and Evolution
13, No. xx, 2013; Pages: 67 - 75

Highlights on molecular identification of closely related species

Lígia A. Almeida, Ricardo Araujo

IPATIMUP, Institute of Molecular Pathology and Immunology, University of Porto, Rua Dr. Roberto Frias s/n, 4200-465 Porto, Portugal.


The term “complex” emerged in the literature at the beginning of the genomic era associated to taxonomy and grouping organisms that belong to different species but exhibited similar patterns according to their morphological, physiological and/or other phenotypic features. DNA–DNA hybridization values 70% and high identity on 16S rRNA gene sequences were recommended for species delineation. Electrophoretic methods showed in some cases to be useful for species identification and population structure but the reproducibility was questionable. Later, the implementation of polyphasic approaches involving phenotypic and molecular methods brought new insights into the analysis of population structure and phylogeny of several “species complexes”, allowing the identification of new closely related species. Likewise, the introduction of multilocus sequence typing and sequencing analysis of several genes offered an evolutionary perspective to the term “species complex”. Several centres worldwide have recently released increasing genetic information on distinct microbial species. A brief review will be presented to highlight the definition of “species complex” for selected microorganisms, mainly the prokaryotic Acinetobacter calcoaceticus –Acinetobacter baumanniiBorrelia burgdorferi sensu lato, Burkholderia cepaciaMycobacterium tuberculosisand Nocardia asteroides complexes, and the eukaryotic Aspergillus fumigatusLeishmania donovani andSaccharomyces sensu stricto complexes. The members of these complexes may show distinct epidemiology, pathogenicity and susceptibility, turning critical their correct identification. Dynamics of prokaryotic and eukaryotic genomes can be very distinct and the term “species complex” should be carefully extended.

Keywords: Bacterial complex; DNA–DNA hybridization; Molecular identification; Multilocus sequence typing; Species definition; Taxonomy



Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution