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Brazilian Journal of Oral Sciences
Vol. 13(1), 2014; Pages: 53-57

In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms

Bruna Paloma de Oliveira , Carla Cabral dos Santos Accioly Lins , Fátima Alves Diniz , Liliane Lima Melo , Célia Maria Machado Barbosa de Castro

Universidade Federal de Pernambuco - UFPE, School of Dentistry, Department of Prosthodontics and Oral Facial Surgery, Recife, PE, Brasil


To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT).

The microorganism indicators were: Candida albicans, Pseudomonas aeruginosa, Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (108 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane's comparisons.

The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001).

PDT was effective in reducing the number of viable cells in the studied microorganisms, especially E. faecalis and S. aureus.

Keywords: endodontics; Enterococcus faecalis; methylene blue; microbiology; photodynamic therapy

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