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Biochemical Engineering Journal
Vol. 85,
2014; Pages: 814

High cell density fed-batch fermentation for the production of a microbial lipase

M.N.I. Salehmin, M.S.M. Annuar, Y. Chisti

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia .


Extracellular lipase of the yeast Candida rugosa was produced via high cell density fed-batch fermentations using palm oil as the sole source of carbon and energy. Feeding strategies consisted of a pH-stat operation, foaming-dependent control and specific growth rate control in different experiments. Compared to foaming-dependent feeding and the pH-stat operation, the specific growth rate control of feeding proved to be the most successful. At the specific growth rate control set at 0.05 h-1, the final lipase activity in the culture broth was the highest at ∼700 U L-1. This was 2.6-fold higher than the final enzyme activity obtained at a specific growth rate control set at 0.15 h-1. The peak enzyme concentration achieved using the best foaming-dependent control of feeding was around 28% of the peak activity attained using the specific growth rate control of feeding at 0.05 h-1. Similarly, the peak enzyme concentration attained using the pH-stat feeding operation was a mere 9% of the peak activity attained by specific growth rate control of feeding at a set-point of 0.05 h-1. Fed-batch fermentations were performed in a 2 L stirred-tank bioreactor (30°C, pH 7) with the dissolved oxygen level controlled at 30% of air saturation.

Keywords: Lipase production; High cell density fed-batch fermentation; pH-stat operation; Specific growth rate control; Palm oil;  Candida rugosa.

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