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Industrial Crops and Products
Volume 177, 2022, 114519

D-Lactic acid production from Cistus ladanifer residues: Co-fermentation of pentoses and hexoses by Escherichia coli JU15

Júnia Alves-Ferreiraa,b,c,d, Florbela Carvalheiroa, Luís C. Duartea

LNEG - Laboratório Nacional de Energia e Geologia, Unidade de Bioenergia e Biorrefinarias, Estrada do Paço do Lumiar, 22, 1649-038 Lisboa, Portugal.


In this study, glucan-rich solids, and xylose-rich hydrolysates obtained from Cistus ladanifer distillery residues (CLR) were used for d-lactic acid (d-LA) production by the d-lactogenic Escherichia coli strain JU15. Firstly, hemicellulosic hydrolysates obtained by the autohydrolysis process were submitted to dilute sulfuric acid-catalysed post-hydrolysis. The influence of operational conditions on oligosaccharides hydrolysis was assessed by the combined severity parameter (CS) in the range of 1.1–2.3. The optimum post-hydrolysis conditions were found for CS of 1.6 (300 mM H2SO4, 15 min, 121 °C). Subsequent detoxification procedures on post hydrolysed liquors were carried out, where 9.1% (w/v) powdered activated charcoal enabled a full removal of furfural, 5-hydroxymethylfurfural (HMF), and phenolic compounds together with a reduction of acetic acid (37%), and formic acid (27%). Diverse fermentation modes using detoxified and non-detoxified hydrolysates, as well as using previously NaOH delignified glucan-rich solids alone (SHF or SSF) or together with pentoses liquors (SSCF) (5% loading) were performed. For all the tested conditions, both hemicellulose- and cellulose-derived sugars can be efficiently used as the carbon source to produce d-lactic acid by E. coli JU15 with a d-LA yield always surpassing 92 gd-LA/100 g sugars.

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