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Bioresource Technology
Vol. 247, 2018, Pages: 267-272

Extraction of intracellular protein from Chlorella pyrenoidosa using a combination of ethanol soaking, enzyme digest, ultrasonication and homogenization techniques

Ruilin Zhang, Jian Chen, Xuewu Zhang

College of Food Science and Engineering, South China University of Technology, Guangzhou, China.


Due to the rigid cell wall of Chlorella species, it is still challenging to effectively extract significant amounts of protein. Mass methods were used for the extraction of intracellular protein from microalgae with biological, mechanical and chemical approaches. In this study, based on comparison of different extraction methods, a new protocol was established to maximize extract amounts of protein, which was involved in ethanol soaking, enzyme digest, ultrasonication and homogenization techniques. Under the optimized conditions, 72.4% of protein was extracted from the microalgae Chlorella pyrenoidosa, which should contribute to the research and development of Chlorella protein in functional food and medicine.

Graphical abstract

Keywords: Chlorella pyrenoidosa, Cell disruption, Protein extraction, Optimization.

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