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Microbial Ecology
Vol. 65, No.
1, 2013; Page: 593 - 601

Changes in Root Bacterial Communities Associated to Two Different Development Stages of Canola (Brassica napus L. varoleifera) Evaluated through Next-Generation Sequencing Technology

Samanta B. de Campos, Jung-Won Youn, Roberto Farina, Sebastian Jaenicke, Sebastian Jünemann, Rafael Szczepanowski, Anelise Beneduzi, Luciano K. Vargas, Alexander Goesmann, Volker F. Wendisch, Luciane M. P. Passaglia

Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Prédio 43312, Sala 207b, Caixa Postal 15.053, Porto Alegre, Rio Grande do Sul, CEP 91501-970, Brazil.

Abstract

Crop production may benefit from plant growth-promoting bacteria. The knowledge on bacterial communities is indispensable in agricultural systems that intend to apply beneficial bacteria to improve plant health and production of crops such as canola. In this work, the diversity of root bacterial communities associated to two different developmental phases of canola (Brassica napusL.) plants was assessed through the application of new generation sequencing technology. Total bacterial DNA was extracted from root samples from two different growth states of canola (rosette and flowering). It could be shown how bacterial communities inside the roots changed with the growing stage of the canola plants. There were differences in the abundance of the genera, family, and even the phyla identified for each sample. While in both root samples Proteobacteria was the most common phylum, at the rosette stage, the most common bacteria belonged to the family Pseudomonadaceae and the genus Pseudomonas, and in the flowering stage, the Xanthomonadaceae family and the genus Xanthomonas dominated the community. This implies in a switch in the predominant bacteria in the different developmental stages of the plant, suggesting that the plant itself interferes with the associated microbial community.

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