Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
Access Statistics

Site Visitors

blog tracking


 
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Vol. 75, No. 15, 2009, Pages: 4993–5000


Quantification of Key Genes Steering the Microbial Nitrogen Cycle in the Rhizosphere of Sorghum Cultivars in Tropical Agroecosystems

Brigitte Hai,1* Ndeye Helene Diallo,2 Saidou Sall,2 Felix Haesler,3 Kristina Schauss,1 Moussa Bonzi,4 Komi Assigbetse,2 Jean-Luc Chotte,5 Jean Charles Munch,3 and Michael Schloter1

Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Department of Terrestrial Ecogenetics, Institute of Soil Ecology, Ingolsta¨dter Landstrasse 1, 85764 Neuherberg, Germany.

Abstract

The effect of agricultural management practices on geochemical cycles in moderate ecosystems is by far better understood than in semiarid regions, where fertilizer availability and climatic conditions are less favorable. We studied the impact of different fertilizer regimens in an agricultural long-term observatory in Burkina Faso at three different plant development stages (early leaf development, flowering, and senescence) of sorghum cultivars. Using real-time PCR, we investigated functional microbial communities involved in key processes of the nitrogen cycle (nitrogen fixation, ammonia oxidation, and denitrification) in the rhizosphere. The results indicate that fertilizer treatments and plant development stages combined with environmental factors affected the abundance of the targeted functional genes in the rhizosphere. While nitrogen-fixing populations dominated the investigated communities when organic fertilizers (manure and straw) were applied, their numbers were comparatively reduced in urea-treated plots. In contrast, ammonia-oxidizing bacteria (AOB) increased not only in absolute numbers but also in relation to the other bacterial groups investigated in the urea-amended plots. Ammonia-oxidizing archaea exhibited higher numbers compared to AOB independent of fertilizer application. Similarly, denitrifiers were also more abundant in the urea-treated plots. Our data imply as well that, more than in moderate regions, water availability might shape microbial communities in the rhizosphere, since low gene abundance data were obtained for all tested genes at the flowering stage, when water availability was very limited.

Keywords:ammonia monooxygenase;Ammonia-oxidizing archaea;Rhizosphere of Sorghum Cultivars;ammonia-oxidizing bacteria.


Corresponding author: Tel 49 89 3187 3479, Fax 49 89 3187 3376.

E-mail: brigitte.hai@helmholtz-muenchen.de

 

 
Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution