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Desalination and Water Treatment
2016, Pages: 1–8

Isolation and characterization of Candida vishwanathii strain TERI MS1 for degradation of petroleum hydrocarbons in marine environment

Anchal Priya, Priyangshu Manab Sarma, Banwari Lal

Bioresources and Biotechnology Division, TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, India.

Abstract

In an ongoing prospecting project for developing a bioremediation strategy for marine oil spills, a strain of yeast was isolated from petroleum crude oil contaminated sample. The strain, TERI MS1 was initially characterized biochemically, subsequently identified as Candida vishwanathii based on the sequence analysis of D1/D2 domain of 26S rRNA, and further confirmed by sequencing of internal transcribed spacer. The strain C. vishwanathii was able to utilize petroleum crude oil in natural seawater with 49 percent degradation in 72 h. The strain has high affinity toward degradation of polyaromatic hydrocarbons with degradation of 83% of pyrene (0.1% w/v) and 69% of naphthalene (0.1% w/v). With the specific growth rate of 1.25 h-1 and doubling time of 33.5 min, TERI MS1 showed potential for its use in implement- ing a bioaugmentation strategy for restoring oil spills in marine environment.

Keywords: Biodegradation; Candida vishwanathii; Petroleum crude oil; Polyaromatic hydrocarbon (PAHs); Seawater.

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