Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
Access Statistics

Site Visitors

blog tracking


 
Applied and Environmental Microbiology
Vol. 72, No: 9, 2006; Pages: 6271-6276


Increase in Terminal Restriction Fragments of Bacteroidetes-Derived 16S rRNA Genes after Administration of Short-Chain Fructooligosaccharides

Yusuke Nakanishi,1, Koichiro Murashima,2*, Hiroki Ohara,2 Takahisa Suzuki,3 Hidenori Hayashi,4 Mitsuo Sakamoto,4 Tomoyuki Fukasawa,2Hidetoshi Kubota,2 Akira Hosono,1 Toshiaki Kono,2 Shuichi Kaminogawa,1 and Yoshimi Benno4

Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd., 5-3-1 Chiyoda, Sakado-shi, Saitama 350-0289, Japan.

Abstract

It is well known that short chain fructooligosaccharides (scFOS) modify intestinal microbiota in animals as well as in humans. Since most murine intestinal bacteria are still uncultured, it is difficult for a culturing method to detect changes in intestinal microbiota after scFOS administration in a mouse model. In this study, we sought markers of positive change in murine intestinal microbiota after scFOS administration using terminal restriction fragment length polymorphism (T-RFLP) analysis, which is a culture-independent method. The T-RFLP profiles showed that six terminal restriction fragments (T-RFs) were significantly increased after scFOS administration. Phylogenetic analysis of the 16S rRNA partial gene sequences of murine fecal bacteria suggested that four of six T-RFs that increased after scFOS administration were derived from the 16S rRNA genes of the class Bacteroidetes. Preliminary quantification of Bacteroidetes by real-time PCR suggests that the 16S rRNA genes derived from Bacteroidetes were increased by scFOS administration. Therefore, the T-RFs derived from Bacteroidetes are good markers of change of murine intestinal microbiota after scFOS administration

Keywords: Terminal Restriction ,Bacteroidetes,16S rRNA Genes,Fructooligosaccharides,T-RFs ,T-RFLP.


Corresponding author: Phone: (81) 492847592. Fax: (81) 492847598.

E-mail: koichiro_murashima{at}meiji.co.jp

 

 
Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution