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Applied and Environmental Microbiology
Vol. 72, No: 9, 2006; Pages: 6271-6276

Increase in Terminal Restriction Fragments of Bacteroidetes-Derived 16S rRNA Genes after Administration of Short-Chain Fructooligosaccharides

Yusuke Nakanishi,1, Koichiro Murashima,2*, Hiroki Ohara,2 Takahisa Suzuki,3 Hidenori Hayashi,4 Mitsuo Sakamoto,4 Tomoyuki Fukasawa,2Hidetoshi Kubota,2 Akira Hosono,1 Toshiaki Kono,2 Shuichi Kaminogawa,1 and Yoshimi Benno4

Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd., 5-3-1 Chiyoda, Sakado-shi, Saitama 350-0289, Japan.


It is well known that short chain fructooligosaccharides (scFOS) modify intestinal microbiota in animals as well as in humans. Since most murine intestinal bacteria are still uncultured, it is difficult for a culturing method to detect changes in intestinal microbiota after scFOS administration in a mouse model. In this study, we sought markers of positive change in murine intestinal microbiota after scFOS administration using terminal restriction fragment length polymorphism (T-RFLP) analysis, which is a culture-independent method. The T-RFLP profiles showed that six terminal restriction fragments (T-RFs) were significantly increased after scFOS administration. Phylogenetic analysis of the 16S rRNA partial gene sequences of murine fecal bacteria suggested that four of six T-RFs that increased after scFOS administration were derived from the 16S rRNA genes of the class Bacteroidetes. Preliminary quantification of Bacteroidetes by real-time PCR suggests that the 16S rRNA genes derived from Bacteroidetes were increased by scFOS administration. Therefore, the T-RFs derived from Bacteroidetes are good markers of change of murine intestinal microbiota after scFOS administration

Keywords: Terminal Restriction ,Bacteroidetes,16S rRNA Genes,Fructooligosaccharides,T-RFs ,T-RFLP.

Corresponding author: Phone: (81) 492847592. Fax: (81) 492847598.

E-mail: koichiro_murashima{at}meiji.co.jp


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