2 1 83
Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
Access Statistics

Site Visitors

blog tracking


 
Chemical Geology
Vol. 391, 2015, Pages: 33–41

Effects of CeO2 nanoparticles on microbial metabolism

Shota Masaki, Hiroyuki Shiotsu, Toshihiko Ohnuki, Fuminori Sakamoto, Satoshi Utsunomiya

Department of Chemistry, Kyushu University, Fukuoka 812-8581, Japan.

Abstract

To understand the effects of nanoparticles on microorganisms, we experimentally investigated the effects of CeO2 nanoparticles (CeNPs) on yeast (S. cerevisiae) focusing on microbial metabolites and intracellular proteins. The yeast were harvested from a yeast extract peptone dextrose medium containing 0, 10, 100, and 250 ppm of CeNPs and incubated for 120 h in 1 mM NaCl solution at three different pH values: 3, 5, and 7. The yeast released organic matter, P, K, and Mg into the NaCl solution at all pH values, even without CeNPs. Distinct differences were detected in the released organic species and intracellular proteins after exposure to CeNPs. High-performance liquid chromatography revealed that various organic species released from the yeast were expressed or suppressed after exposure to CeNPs. Although cytotoxicity was not caused by CeNPs, the results of the peptide mass fingerprint analysis of the intracellular protein revealed that Eno2p, a glycolysis enzyme, was expressed after exposure to CeNPs. These results suggest that nanoparticles have the potential to alter microbial metabolism, leading to changes in the compositions of the released substances in the surrounding environment.

Keywords: CeO2 nanoparticle; Microorganism; Organic matter; Metabolism.


 
Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution