Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
mn

Site Visitors

blog tracking


 
Applied Soil Ecology
Vol.
63, 2013; Pages: 15 - 22

Screening of rhizobacteria isolated from maize (Zea mays L.) in Rio Grande do Sul State (South Brazil) and analysis of their potential to improve plant growth

Letícia Arruda, Anelise Beneduzi, Adriana Martins, Bruno Lisboa, Cristiane Lopes, Fernanda Bertolo, Luciane Maria P. Passaglia, Luciano K. Vargas

Fundação Estadual de Pesquisa em Agropecuária (FEPAGRO), Rua Gonçalves Dias, 570, CEP 90130-060, Porto Alegre, RS, Brazil.

Abstract

Plant growth-promoting rhizobacteria (PGPR) are considered to have a beneficial effect on host plants and may facilitate plant growth by different mechanisms. In this work, the influence of different soil types on the bacterial diversity and the stimulatory effects of selected PGPR on two cultivars of maize were investigated. A set of 292 strains was isolated from the roots and rhizosphere soil of maize cultivated in five different areas of the Rio Grande do Sul State in Brazil. 16S rDNA-PCR-RFLP and 16S rDNA partial sequencing were used for identification, and the Shannon–Weaver index was used to evaluate bacterial diversity. We evaluated the ability of each isolate to produce indole acetic acid (IAA), siderophores and solubilize phosphates. On the basis of multiple PGP traits, six isolates were selected to test their potential as plant growth-promoting rhizobacteria on maize plants. In both the roots and the rhizospheric soil of maize, the dominant bacterial genera identified were Klebsiella and Burkholderia. IAA producers were distributed widely among isolates, regardless of the sampling site. Approximately 42% of the isolates exhibited at least two attributes, and 24% showed all three PGP traits. Three strains, identified as AchromobacterBurkholderia, and Arthrobacter, were effective as PGPR in both of the cultivars evaluated.

Keywords: Maize; PGPR; IAA; Bacterial diversity; Siderophore; Phosphate solubilization


 

 

 
Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution