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Int. J. Mol. Sci
Vol. 14, No. xx, 2013; Pages: 11113-11124

The Interaction of CuS and Halothiobacillus HT1 Biofilm in Microscale Using Synchrotron Radiation-Based Techniques

Huirong Lin1,2, Guangcun Chen1, Shenhai Zhu1, Yingxu Chen1, Dongliang Chen3, Wei Xu3, Xiaohan Yu4 and Jiyan Shi1,*

Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.


In order to investigate the microbe-mineral interaction in the micro scale, spatial distribution and speciation of Cu and S in Halothiobacillus HT1 biofilm formed on a CuS surface was examined using synchrotron-based X-ray techniques. Confocal laser scanning microscope (CLSM) results indicated that Halothiobacillus HT1 biofilm formation gave rise to distinct chemical and redox gradients, leading to diverse niches in the biofilm. Live cells were distributed at the air-biofilm and membrane-biofilm interface. CuS was oxidized by Halothiobacillus HT1 biofilm, and copper penetrated into the biofilm. Sulfide was oxidized to cysteine (77.3%), sulfite (3.8%) and sulfonate (18.9%). Cu-cysteine-like species were involved in the copper homeostasis. These results significantly improve our understanding of the interfacial properties of the biofilm-mineral interface.

Keywords:Halothiobacillus HT1 biofilm; distribution; speciation; copper; sulfur.

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