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Food Control
Volume 89, 2018, Pages 260-269

Synergistic effect of essential oils and enterocin KT2W2G on the growth of spoilage microorganisms isolated from spoiled banana peel

Imanol Tubia, Karthik Prasad, Eva Pérez-Lorenzo, Cristina Abadín, Miren Zumárraga, Iñigo Oyanguren, Francisca Barbero, Jacobo Paredes, Sergio Arana

Ceit, Manuel Lardizabal 15, 20018 Donostia/San Sebastián, Spain.


Spoilage yeasts detection is the key to improve the quality of alcoholic fermentation beverages such as wine and cider. The metabolic activity of the spoilage yeast causes irreparable damage to many liters of final products every year. Therefore, winemakers and cider-house companies suffer a substantial economic impact. Thus, over the years, many detection techniques have been proposed to control the occurrence of spoilage yeast.

Out of the many spoilage yeast genera, Brettanomyces is one of the most commonly encountered in the beverage industry. Leveraging its ability to thrive in wine and cider conditions (low pH, high levels of ethanol, and low oxygenation levels), Brettanomyces can proliferate inside beverage production tanks. Moreover, their resultant by products reduce the quality of the beverage. While the beverage industry has made great strides in detecting harmful organisms, gaps remain.

Traditional methods such as microscopy, cell plating, gas chromatography–mass spectrometry, etc. are often imprecise, expensive, and/or complicated. New emerging spoilage yeast detection platforms, such as biosensors and microfluidic devices, aim to alleviate these constraints. Novel platforms have already demonstrated great promise to be a real alternative for in situ and fast detection in the beverage industry. Finally, the review discusses the potential of emerging spoilage yeast detection and treatment methods.

Keywords: Spoilage yeasts, Brettanomyces, Alcoholic beverages, Detection methods, Control methods, Biosensors.

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