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192, No. 2, 2011; Pages: 584–585

Genome Sequence of the Marine Janibacter Sp. Strain HTCC2649

J. Cameron Thrash,1† Jang-Cheon Cho,2† Anthony D. Bertagnolli,1 Steve Ferriera,3 Justin Johnson,3 Kevin L. Vergin,1 and Stephen J. Giovannoni1*

Department of Microbiology, Oregon State University, Corvallis, OR 97331.


Janibacter sp. strain HTCC2649 is a novel marine member of the Actinobacteria, family Intrasporangiaceae, and is closely related to Janibacter melonis CM2104T and Knoellia sinensis HKI 0119T. The organism was isolated from a sample collected at Hydrostation S south of Bermuda by using high-throughput culturing techniques. Here we present the genome sequence of Janibacter sp. strain HTCC2649. Ongoing high-throughput culturing (HTC) studies to isolate and characterize novel bacterioplankton resulted in the cultivation of a novel marine Actinobacteria, Janibacter sp. strain HTCC2649. Distinct from the more abundant, and as-yet-uncultured, canonical and monophyletic “marine Actinobacteria” clade (OM1 clade) often recovered in culture-independent studies (2, 3, 8), HTCC2649 is more closely related to Janibacter melonis CM2104T (13) and Knoellia sinensis HKI 0119T (5), even though these three strains are found in very different habitats. Water collected at Hydrostation S, 12 miles southeast of Bermuda (see reference 10), from a depth of 10 m, was used to inoculate low-nutrient heterotrophic medium (LNHM) with amended mixed carbon compounds (9). After initial cultivation using previously described HTC techniques (1, 11), the dilution culture was plated on marine agar 2216. HTCC2649 was isolated as a single colony and transferred into liquid medium. The organism was selected for sequencing due to the unique phylogenetic position among the Actinobacteria.

Keywords: Janibacter sp.;Actinobacteria;Intrasporangiaceae;Janibacter melonis;Knoellia sinensis.

Corresponding author: Tel (541) 737-1835, Fax (541) 737-0496.

E-mail: steve.giovannoni@oregonstate.edu


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