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192, No. 23, 2010; Pages: 6315–6316

Genome Sequence of the Marine Alphaproteobacterium HTCC2150, Assigned to the Roseobacter Clade

Ilnam Kang,1 Hyun-Myung Oh,1 Kevin L. Vergin,2 Stephen J. Giovannoni,2 and Jang-Cheon Cho1*

Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.


Here we announce the genome sequence of a marine bacterium, HTCC2150, that was isolated off the Oregon coast using dilution-to-extinction culturing and that is affiliated with the Roseobacter clade. The 16S rRNA phylogeny showed that the strain was closely related to members of the RCA clade. The genome sequence suggests that strain HTCC2150 is an organoheterotroph carrying diverse metabolic potential, including a close relationship with phytoplankton. The Roseobacter clade in the order Rhodobacterales constitutes major alphaproteobacterial lineages in the pelagic ocean together with the SAR11 and SAR116 groups (2, 8). Owing to the well-developed polyphasic taxonomic approach, many novel genera, including Oceanicola, Maribius, and Maritimibacter, have recently been established, exceeding at least 50 genera in this clade (http://www.bacterio.cict.fr). Physiological and genomic analyses of these genera showed that members of the Roseobacter clade are phenotypically diverse and functionally heterogeneous, being associated with, e.g., aerobic anoxygenic photoheterotrophy, aerobic sulfite oxidation, methylotrophy, demethylation of dimethylsulfoniopropionate (DMSP), degradation of aromatic compounds, exopolysaccharide production, and rosette formation (9, 10, 13, 14). All of the validly published genera in this clade form colonies on nutrient agar media, such as marine agar 2216. In our continuing studies using dilution-toextinction culturing (4, 18), however, many novel isolates belonging to several subclades (OM42, NAC11-7, CHAB-I-V, etc.) in the Roseobacter clade did not produce colonies upon initial isolation, which made polyphasic characterization extremely difficult. Here we report the cultivation and draft genome sequence of strain HTCC2150, a new member of the Roseobacter clade that did not form colonies on nutrient agar medium.

Keywords: Roseobacter clade;Rhodobacterales;Oceanicola; Maribius;Maritimibacter;Marine Alphaproteobacterium.

Corresponding author: Tel 82-32-860-7711, Fax 82-32-232-0541

E-mail: chojc@inha.ac.kr


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