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APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Vol.75, No.
8, 2009; Pages: 2581–2584

Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals

Raju Sekar,* Longin T. Kaczmarsky, and Laurie L. Richardson

Biological and Environmental Systems Group, Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom.

Abstract

Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

Keywords: Marine Microbes,16S rRNA Genes,Alphaproteobacteria,Escherichia coli, marine biodiversity.


Corresponding author: Tel 44 114 222 0062. Fax: 44 114 222 7501.

E-mail: S.Raju@Sheffield.ac.uk

 

 
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