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Sensors and Actuators B: Chemical
Volume 346, 2021, 130549

MXene catalyzed Faraday cage-type electrochemiluminescence immunosensor for the detection of genetically modified crops

Xiaoshuang Chen, Dongyu Zhang, Han Lin, Wenting Wei, Tingting Hao, Yufang Hu, Sui Wang, Zhiyong Guo

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, PR China.

Abstract

Herein, a MXene catalyzed Faraday cage-type electrochemiluminescence (ECL) immunosensor was developed for the detection of transgenic component Cry1Ab protein in genetically modified (GM) crops. The capture unit Fe3O4-Ab1 is Fe3O4 nanoparticles coated by capture antibody Ab1, while the signal unit MXene-PTCA-Ab2 is two-dimensional conductive material MXene with ECL labels 3,4,9,10-perylenetetracarboxylic acid (PTCA) and recognition antibody Ab2 simultaneously immobilized. In presence of the target Cry1Ab, the Faraday cage-type electrochemical immunosensor could be constructed by forming the capture unit-Cry1Ab-signal unit immunocomplex. In addition to the inherently high sensitivity brought by the Faraday cage-type sensor construction mode, MXene in the signal unit catalyzes the reduction of dissolved O2 to form reactive oxygen species which could accelerate the ECL reaction, resulting in a secondary ECL enhancement of PTCA and ensuing higher detection sensitivity. Under the optimized experimental conditions, Cry1Ab protein and GM maize MON810 could be quantitatively detected in the range of 0.005 ∼ 100 ng mL−1 and 0.005 % ∼ 2.0 %, with limit of detections (LODs) 0.001 ng mL−1 and 0.001 % respectively. Selectivity, stability, repeatability, precision, and accuracy are all satisfactory. Detection of actual samples is successful, showing its application prospect in the field of agriculture and food safety.

Keywords: Genetically modified (GM) crops, Cry1Ab, MXene, 3,4,9,10-perylenetetracarboxylic acid (PTCA), Electrochemiluminescence (ECL), Faraday cage-type immunosensor.

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