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Capillary Gel Electrophoresis
2022, Pages 351-363

Related microseparation techniques

András Guttman, László Hajba

Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.

Abstract

Capillary electrophoresis-related microseparation techniques include ultrathin-layer gel electrophoresis and microchip gel electrophoresis. Since this latter can be a subject of another book, in this chapter we only discuss the former one. Analysis of DNA molecules was accomplished by ultrathin-layer gel electrophoresis in early sequencing applications and fragment analysis. Ultrathin gels were also utilized in multilane separation of sodium dodecyl sulfate–protein complexes, but showed their real potential in two-dimensional gel electrophoresis.

Keywords: 2D electrophoresis, isoelectric focusing, size separation, proteins, staining.

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