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Antimicrobial Dressings
2023, 113-131

Different methods for nanomaterial-based immobilization of enzymes

Satyabrat Gogoi1, Jejiron M. Baruah2, Geetanjali Baruah3

Department of Chemistry, School of Basic Sciences, The Assam Kaziranga University, Jorhat, Assam, India.

Abstract

Immobilization of enzymes has received a lot of attention in recent years because of its numerous potential applications in industrial and manufacturing processes such as biomedicine, antimicrobial, and catalytic applications for chemical synthesis. Immobilized enzymes have several advantages over native mobile enzymes, including reusability, increased thermal and operational stability, rapid reaction termination, and controlled product formation. Microwave-assisted immobilization, cross-linked enzyme aggregates, mesoporous supports, click chemistry technology, and, most recently, nanoparticle (NP)-based immobilization of enzymes have all been used to prepare immobilized enzymes. Because NPs have a high surface-to-volume ratio, the concentration of the immobilized enzyme is significantly higher in NP-based immobilization compared to other techniques based on immobilization on planar 2-D surfaces. This chapter discusses the various NPs used for enzyme immobilization, as well as the methods for preparing NP-based enzyme immobilization.

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