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Journal of Chromatography A
Volume 1638, 2021, 461882

Determination of activity of denitrifying enzyme in soil samples by a headspace gas chromatographic technique

Xu-Bo Zhanga, Zhan-BoHua, Xin-Sheng Chaib, Guang-Zhi Zhongc, Xiao Ouyanga, Pei-Yi Lia

School of Resources, Environment and Materials, Guangxi University, Nanning, China.


This paper reported a headspace gas chromatography (HS-GC) for the determination of denitrifying enzyme activity in soil samples. It was based on measuring the NO2 signal in a set of closed/air-free vials containing soil samples that incubated at the designated conditions. The results showed that the method has a good measurement precision (RSD < 5.0%) and reasonable accuracy for determining the denitrifying enzyme activity in soil samples. It is simple and efficient, and very suitable to be used in batch sample analysis.

Keywords: Headspace Gas Chromatography, Denitrifying enzyme, Nitrite nitrogen, Nitrogen dioxide.

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