Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
Access Statistics

Site Visitors

blog tracking


 
Biochemical Systematics and Ecology
Volume 93, 2020, 104140

Comparative analysis of the constituents of two cultivars of Dioscorea dumetorum (Kunth) Pax. and their molecular barcoding

Adedapo Adedayo Adenirana,b,c, Mubo Adeola Sonibarea, ShashiKumarc

Department of Pharmacognosy, University of Ibadan, Ibadan, Nigeria.

Abstract

The edible and wild cultivars of Dioscorea dumetorum (Kunth) Pax. (Family: Dioscoreaceae) are commonly used as close substitutes in herbal markets due to their edibility and medicinal importance. Comparative study of the cultivars was done using chemical analysis of their methanol tuber extracts and by utilizing the DNA barcode regions, ribulose-1, 5-bisphosphate carboxylase subunits (rbcL) and the non-coding intergenic spacer gene (trnH-psbA) for nucleotide sequences comparison of cultivars. Of the 54 compounds identified from the methanol extract of the edible and wild cultivars using gas chromatography-mass spectrometry (GC-MS), 12 are similar, while 42 are remarkably different. In particular n-hexadecanoic (palmitic) acid had a higher percentage area in the edible cultivar (31.16%) compared to the wild cultivar (0.26%). The cultivars were successfully amplified using the universal primers gene rbcL and trnH-psbA. Their nucleotide sequences showed a slight variation when aligned with CodonCode Aligner V.9.0.1. They were identified by the Basic Local Alignment Search Tool (BLAST) through comparison with Genbank data, which shows close similarities with submitted data. The use of molecular barcoding in identifying the cultivars of Dioscorea dumetorum as well as the chemical composition of their tubers may form an important marker in the compilation of future herbal pharmacopoeia for its proper identification.

Keywords: Cultivar, Chemical composition, Dioscorea dumetorum, DNA barcode, Gas chromatography-mass spectrometry.

Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution