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Bioresource Technology
138, 2013; Pages: 222 - 234

Evaluation of Pseudomonas aeruginosa an innovative bioremediation tool in multi metals ions from simulated system using multi response methodology

Rajesh Singh, Narsi R. Bishnoi, Anita Kirrolia

Department of Environmental Science & Engineering, Guru Jambheshwar University of Science & Technology, Hisar, Haryana 125001, India


Under certain conditions bacteria can act as a good biosorbent towards heavy metals in simultaneous removal from effluents. The present study explores overlay plots of multi response surface methodology for simulated wastewater treatment potential. Pseudomonas aeruginosa was used for bioremediation of metallic ions, where removal of Cd (80–90%), Mn (85–90%), Fe (50–55%), Cr (70–75%) can be achieved by fixing the pH, oxidation reduction potential (mV) and one of the metallic constituent in the simulated effluent. The metal ions Cd and Cr (T), Fe and ORP (mV) are relatively closely located to each other in the loading plot indicating co-variance between these components. However Cr(VI) transformation and Mn removal are distantly placed in the bi-plot indicating the existed significant difference. Elevated reductase enzyme activity (31.75 μg/min mg) observed in the isolate showing the ability to effectively reduce metals ions.

Keywords: Simulated effluent; Pseudomonas aeruginosa; Multi response surface methodology; Principal component analysis; Oxidation reduction potential



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