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Bioresource Technology
Vol. 168, 2014, Page: 136 - 141

Enzymatic hydrolysis and characterization of waste lignocellulosic biomass produced after dye bioremediation under solid state fermentation

Pankajkumar R. Waghmare, Avinash A. Kadam, Ganesh D. Saratale, Sanjay P. Govindwar

Department of Biochemistry, Shivaji University, Kolhapur 416004, India.


Sugarcane bagasse (SCB) adsorbes 60% Reactive Blue172 (RB172). Providensia staurti EbtSPG able to decolorize SCB adsorbed RB172 up to 99% under solid state fermentation (SSF). The enzymatic saccharification efficiency of waste biomass after bioremediation of RB172 process (ddSCB) has been evaluated. The cellulolyitc crude enzyme produced by Phanerochaete chrysosporium used for enzymatic hydrolysis of native SCB and ddSCB which produces 0.08 and 0.3 g/L of reducing sugars respectively after 48 h of incubation. The production of hexose and pentose sugars during hydrolysis was confirmed by HPTLC. The effect of enzymatic hydrolysis on SCB and ddSCB has been evaluated by FTIR, XRD and SEM analysis. Thus, during dye biodegradation under SSF causes biological pretreatment of SCB which significantly enhanced its enzymatic saccharification. Adsorption of dye on SCB, its bioremediation under SSF produces wastes biomass and which further utilized for enzymatic saccharification for biofuel production.

Keywords: Bioremediation; Solid state fermentation; Lignocellulosic biomass; Enzymatic hydrolysis; Phanerochaete chrysosporium



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