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Journal of Microbiological Methods
Vol. 68,No: xx, 2007, Pages: 437–441

A novel and in situ technique for the quantitative detection of MTBE and benzene degrading bacteria in contaminated matrices

J.P. Biggerstaffa,*, M. Le Puilb, B.L. Weidowa, J. Leblanc-Gridleya, E. Jenningsc, J. Busch-Harrisc, K.L. Sublettec, D.C. Whitea, R.S. Alberteb

University of Tennessee, Knoxville, TN, United States.


A novel and in situ technique is presented here as a better alternative to culture-dependent and PCR-based techniques for the quantitative detection of predominant bacterial species involved in the bioremediation of contaminants. It allowed rapid, specific and in situ identification of Biosep®-immobilized eubacteria from MTBE- and benzene-contaminated matrices.

Keywords: MTBE, Fuel oxygenate, Bioremediation, FISH, Spectral imaging,methyl tertbutyl
ether,tert-butyl alcohol,microorganisms,bioremediation.

Corresponding author: Tel +1 865 974 8021; fax +1 865 974 8027

E-mail: jbiggers@utk.edu


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