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Bioresource Technology
Vol. 175, 2015, Pages: 358–366

Bioprocess development for the production of sonorensin by Bacillus sonorensis MT93 and its application as a food preservative

Lipsy Chopra, Gurdeep Singh, Kautilya Kumar Jena, Himanshu Verma, Debendra K. Sahoo

Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, India.


Media composition and environmental conditions were optimized using statistical tools, Plackett Burman design and response surface methodology, to maximize the yield of a bacteriocin, named as sonorensin, from a new marine isolate Bacillus sonorensis MT93 showing broad spectrum of antimicrobial activity. Under optimized conditions, MT93 produced 15-fold higher yield of sonorensin compared to that under initial fermentation conditions. As oxygen supply is a critical parameter controlling growth and product formation in aerobic bioprocesses and used as a parameter for bioprocess scale up, the effects of oxygen transfer, in terms of volumetric oxygen transfer coefficient (kLa), on production of sonorensin was investigated using optimized medium composition in a bioreactor. Studies on effectiveness of sonorensin against Staphylococcus aureus and Listeria monocytogenes in fruit juice and as a preservative in pasteurized milk demonstrated its potential as a biopreservative in fruit products and shelf life extender of the pasteurized milk.

Keywords: Bacillus sonorensis; Sonorensin; Optimization; Volumetric oxygen transfer coefficient; Biopreservative.

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