Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Research on Microbes
Microbiology Experts
Online Submission
Access Statistics

Site Visitors

blog tracking

Marine Pollution Bulletin
62, No. 5, 2011; Pages: 1032 - 1040

Antifouling activity of commercial biocides vs. natural and natural-derived products assessed by marine bacteria adhesion bioassay

Mercedes Camps, Jean-François Briand, Linda Guentas-Dombrowsky, Gérald Culioli, Alexis Bazire, Yves Blache

Laboratoire MAPIEM, EA 4323, Biofouling et Substances Naturelles Marines, Université du Sud Toulon-Var, 83162 La Valette-du-Var, France.


Biofilm formation is a key step during marine biofouling, the natural colonization of immersed substrata, leading to major economic and ecological consequences. Consequently, bacteria have been used for the screening of new non-toxic antifoulants: the adhesion of five strains isolated on three French locations was monitored using a fluorescence-based assay and toxicity was also evaluated. Nine biocides including commercial, natural and natural-derived products were tested. The commercial antifoulants, TBTO and Sea Nine showed low EC50 but high toxicity. The non-commercial products TFA-Z showed significant anti-adhesion activities and appeared to be non-toxic, suggesting a specific anti-adhesion mechanism. In addition, the strains could be classified depending on their sensitivity to the molecules used even if strain sensitivity also depended on the molecules tested. In conclusion, TFA-Z would be a promising candidate as non-toxic antifoulant and our results strengthen the need to perform antifouling bioassays with a panel of strains showing different response profiles.

Keywords: Biofouling; Biofilm; Microtiter plate; Fluorescence; Natural products; Commercial biocides



Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution