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Acta Petrolei Sinica(Petroleum Processing Section)
xx, No. xx, 2011; Pages: xxx - xxx

Analysis of Bacterial Community Diversity of Biofouling in Recirculating Cooling Water System by Using PCR-DGGE

MA Tao,LIU Fang,ZHAO Chaocheng,ZHANG Pei

College of Chemistry and Chemical Engineering,China University of Petroleum,Qingdao 266555,China.


The recirculating cooling water systems at three different COD values of 25,50 and 100 mg/L were used for vaccination water to investigate the bacterial count variations and microbial community composition of biofouling by polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE) analysis.The experimental results showed that the maximum bacteria count was found in the biofouling at moderate nutrient level(COD=50 mg/L) rather than abundant nutrient level(COD=100 mg/L).Both the nutrient level and consequent biofouling structure were the important factors affecting the amount of biofouling bacteria.The diversity of biofouling bacteria community decreased with inoculation COD value of 25,50 and 100 mg/L in turn.Furthermore,the resemblance of bacterial community structure in biofouling at abundant nutrient level was demonstrated to significantly differ from the other two samples by Jaccard coefficient and clustering analysis.In addition,19 main bands in DGGE profile were further selected,cloned and sequenced.Based on similarity matching,all the sequences showed higher than 97% similarity to relative registered DNA sequences of bacteria strains in GenBank.There were two distinct groups of preponderant bacteria strains.Although pair groups of them were closely related to each other,they showed different predominant position in biofouling at different nutrient levels.

Keywords: recirculating cooling water biofouling bacterial community diversity PCR-DGGE technology



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