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Journal of Hazardous Materials
Vol. 313, 2016, Pages: 170–178

The peroxidase-mediated biodegradation of petroleum hydrocarbons in a H2O2-induced SBR using in-situ production of peroxidase: Biodegradation experiments and bacterial identification

Sakine Shekoohiyan, Gholamreza Moussavi, Kazem Naddafi

Department of Environmental Health Engineering, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.


A bacterial peroxidase-mediated oxidizing process was developed for biodegrading total petroleum hydrocarbons (TPH) in a sequencing batch reactor (SBR). Almost complete biodegradation (>99%) of high TPH concentrations (4 g/L) was attained in the bioreactor with a low amount (0.6 mM) of H2O2 at a reaction time of 22 h. A specific TPH biodegradation rate as high as 44.3 mgTPH/gbiomass×h  was obtained with this process. The reaction times required for complete biodegradation of TPH concentrations of 1, 2, 3, and 4 g/L were 21, 22, 28, and 30 h, respectively. The catalytic activity of hydrocarbon catalyzing peroxidase was determined to be 1.48 U/mL biomass. The biodegradation of TPH in seawater was similar to that in fresh media (no salt). A mixture of bacteria capable of peroxidase synthesis and hydrocarbon biodegradation includingPseudomonas spp. and Bacillus spp. were identified in the bioreactor. The GC/MS analysis of the effluent indicated that all classes of hydrocarbons could be well-degraded in the H2O2-induced SBR. Accordingly, the peroxidase-mediated process is a promising method for efficiently biodegrading concentrated TPH-laden saline wastewater.

Keywords: Petroleum hydrocarbon; Biodegradation; Bioreactor; Enzyme; Peroxidase; Salinity.
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