Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Research on Microbes
Microbiology Experts
Online Submission
Access Statistics

Site Visitors

blog tracking

Organic Geochemistry
Vol. 97, 2016, Pages: 1–4

Tracking the signals of living archaea: A multiple reaction monitoring (MRM) method for detection of trace amounts of intact polar lipids from the natural environment

Yufei Chen, Chuanlun Zhang, Chengling Jia, Fengfeng Zheng, Chun Zhu

State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, China.


Isoprenoid glycerol dialkyl glycerol tetraethers (iGDGTs) are biomarkers of archaea, but improvement is needed for more precise analysis of intact polar lipid (IPL)-iGDGTs. Here, we present a multiple reaction monitoring (MRM) method for IPL-iGDGTs using reversed phase liquid chromatography (RP-LC) and triple quadrupole mass spectrometry equipped with electrospray ionization (RP-LC–ESI-MRM). The MRM mode showed much greater sensitivity and lower detection limits than the commonly used selected ion monitoring (SIM) mode. Furthermore, the MRM mode had better stability and repeatability than the SIM mode. Thus, the RP-LC–ESI-MRM method can allow efficient fingerprinting of archaeal IPL-iGDGTs, particularly when they are in trace abundance in natural environments.

Keywords: IPL-iGDGTs; Living archaea; RP-LC–ESI-MRM.

Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution