Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Research on Microbes
Microbiology Experts
Online Submission
Access Statistics

Site Visitors

blog tracking

Electronic Journal of Biotechnology
Vol. 25, 2017, Pages: 58–63

Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring

Emky Valdebenito-Rolack, Nathaly Ruiz-Tagle, Leslie Abarzúa, Germán Aroca, Homero Urrutia

Laboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, Chile.



Sulphur-oxidizing microorganisms are widely used in the biofiltration of total reduced sulphur compounds (odorous and neurotoxic) produced by industries such as the cellulose and petrochemical industries, which include high-temperature process steps. Some hyperthermophilic microorganisms have the capability to oxidize these compounds at high temperatures (> 60°C), and archaea of this group, for example, Sulfolobus metallicus, are commonly used in biofiltration technology.


In this study, a hyperthermophilic sulphur-oxidizing strain of archaea was isolated from a hot spring (Chillán, Chile) and designated as M1. It was identified as archaea of the genus Sulfolobus (99% homology with S. solfataricus 16S rDNA). Biofilms of this culture grown on polyethylene rings showed an elemental sulphur oxidation rate of 95.15 ± 15.39 mg S l-1 d-1, higher than the rate exhibited by the biofilm of the sulphur-oxidizing archaea S. metallicus (56.8 ± 10.91 mg l-1 d-1).


The results suggest that the culture M1 is useful for the biofiltration of total reduced sulphur gases at high temperatures and for other biotechnological applications.

Keywords: Biofilms on polyethylene; Biofiltration; Cellulose industries; Denaturing gradient gel electrophoresis extremophile; Hyperthermophile; Industrial gas emissions; Petroleum refinery; Sulfolobus; Sulphide; Sulphur-oxidizing archaea; Sulphur-oxidizing microorganisms.

Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution