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Patents

Materials and Methods for Assessing and Mapping Microbes and Microbial Biofilms on Wounds

       The subject invention provides point-of-care assays for assessing the topographical distribution of microbial biofilm and/or specific microorganisms in wounds.

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Fibrous web comprising a cationic polymer for capturing microorganisms

       A fibrous web that includes a cationic polymer that contains a cationic monomer comprising a cationic functional group having an affinity for the negatively charged cell walls of bacteria and a hydrophobic monomer comprising a hydrophobic functional group is provided. The molar ratio of the cationic monomer to the hydrophobic monomer is greater than about 1:1. The affinity of the cationic polymer for the bacteria allows the web to capture bacteria, thereby removing them from a surface or liquid, without the use of harsh chemicals, and also inhibiting their spread to other surfaces and liquids that may contact the web.

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Lactobacillus johnsonii CNCM I-1225 for the anti-adhesion of skin pathogenic flora

       Bacterial agents for preparing compositions which are for cosmetic, pharmaceutical or veterinary use and which are intended to stabilize and/or regulate the cutaneous ecosystem of mammals. These bacterial agents being an extract of a bacterium, or a bacterium, selected for their adhesion to skin cells and anti-adhesive to pathogens of the cutaneous system. The invention also relates to compositions containing such agents.

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Compositions containing amino acids, phosphate and manganese and their uses

       The invention provides methods of producing vaccines directed against microorganisms, with the methods comprising culturing, harvesting and/or suspending the microorganism in the presence of a radiation-protective composition and irradiating the bacteria or viruses with a dose of radiation sufficient to render the microorganism replication-deficient and/or non-infective. The radiation-protective compositions used in the methods of the present invention comprise at least one nucleoside, at least one antioxidant and at least one small peptide. The invention also provides methods of rendering bacteria in culture resistant to ionizing radiation (IR), with these methods comprising culturing the bacteria in the presence of a radiation-protective composition.

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Plasticizers made from oil extracted from microorganisms and polar polymeric compositions comprising the same

       Plasticizers are made from oil with a narrowed, fatty acid polydispersity and extracted from a microorganism, such as a natural or genetically modified bacterium or algae. These plasticizers can comprise either a large content of either saturated C4 and/or C6 triglycerides or unsaturated C12 or greater triglycerides that have been chemically modified by one or more of epoxidation, acylation and esterification. The plasticizers of this invention are particularly well-suited for use with polar polymeric resins such as PVC.

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Pentose fermentation by a recombinant microorganism

       The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.

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Microorganism detection method

       Provided is a microorganism detection method which is highly versatile and applicable to both the test for the detection of specified microorganisms and the microbial enumeration test under the same test conditions for Kampo extract preparations and crude drugs containing a variety of antimicrobial substances, is able to effectively reduce the influence of antimicrobial substances in a test sample, and may detect target microorganisms with high precision. This method is a microorganism detection method in a test sample having antimicrobial activity, including a step of preparing a sample liquid containing a test sample having antimicrobial activity and a step of culturing a target microorganism in a culture medium, the method including allowing a (meth) acrylic acid ester-based synthetic adsorbent to act on the sample liquid and/or the culture medium.

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Method of production of granulated micelle-clay complexes: application for removal of organic, inorganic anionic pollutants and microorganisms from contaminated water

       The present invention pertains to a complex comprising micelles of organic cation adsorbed on clay in granulated form, to a method for obtaining an aqueous solution substantially free of organic, inorganic anionic pollutants, or microorganisms present therein, comprising contacting the aqueous solution containing said pollutants with such a complex and to a system for obtaining an aqueous solution substantially free of organic, inorganic anionic pollutants, or microorganisms present therein employing the granulated complex.

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Process for producing L-5-methoxytryptophan using microorganisms

       1-5-Methoxytryptophan, a material for 5-hydroxytryptophan which is useful as an antidepressant and has hormonal actions against Down's syndrome and the like, is produced by culturing a microorganism, which can produce L-5-lower alkoxytryptophan, in a culture medium containing 5-lower alkoxyindole, together with or without serine, in addition to a carbon source, a nitrogen source, inorganic salts and nutrients.

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Microorganism genomics, compositions and methods related thereto

       The present invention provides methods and compositions for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways.

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Microorganisms and method for producing xylitol or d-xylulose

       According to the present invention, there are provided microorganisms having an ability to producing xylitol or D-xylulose by fermentation, and a method for producing xylitol or D-xylulose using the microorganisms. Osmophilic microorganisms were collected from soil, and the obtained microorganisms were searched for a bacterium having an ability to produce xylitol or D-xylulose from glucose. Xylitol or D-xylulose is produced by culturing an isolated bacterium in a suitable medium to accumulate xylitol or D-xylulose in the medium, and collecting xylitol or D-xylulose from the medium.

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Method for production of arachidonic acid

       The present invention relates to a process for producing arachidonic acid. In one embodiment, Mortierella sect. schmuckeri microorganisms are cultured in fermentation medium, preferably containing a component of a complex nitrogen source. Further disclosed is a food product which includes Mortierella sect. schmuckeri microorganisms or lipid isolated from such microorganisms to enhance the arachidonic acid content of the food product.

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Patenting of Microorganisms

       For the first time in India perhaps in any developing country, a comprehensive report on Patenting of Microorganisms has been prepared by the Patent Facilitating Centre. Ever since the US Supreme Court allowed the patenting of microorganisms in 1980, this subject has been drawing a great deal of attention all over the world. As microorganisms are important constituents of biodiversity, issues like the origin of a microorganism and its patentability and ownership have gained importance.

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Device and method for detecting microorganisms

       An instrument and a sealable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium communicating with a sensor means containing an indicator medium therein. Changes in the indicator medium resulting from pH change or change in CO2 concentration in the medium are detected from outside the vessel.

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Transformation vector comprising transposon, microorganisms transformed with the vector, and method for producing L-lysine using the microorganism

       The present invention relates to a transformation vector comprising the partial fragments of a gene encoding transposase, a microorganism transformed with the vector, and a method of producing lysine using the microorganism.

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Device for detecting microorganisms

       An apparatus and method for mixing/agitating microorganism culture bottles includes a wheel in which culture bottles are placed, with the wheel being set at an angle from vertical so that bottles are not inverted during rotation. A sensor such as a florescence or colorimetric sensor for carbon dioxide is disposed along the flat bottom surface of each of the bottles. Once during each revolution the bottles pass across a photodiode/light emitting diode pair (for a calorimetric system). A sensor transmits light emitted by the LED to the photodiode. At the same instant, an infrared reflective objective sensor, emitting on a digital rotary encoder, determines the bottle number. The change in sensor transmittance is directly related to cell growth so that bottles positive for growth can be detected by suitable software.

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Method of producing coenzyme q10 by microorganisms

       Certain yeasts and bacteria produce a large amount of intracellular coenzyme Q10 which is useful as medicine.

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Measurement of gas production of microorganisms

       A simple apparatus and method is disclosed for measuring gas production by microorganisms using a pressure transducer to sense pressure buildup by members of the Enterobacteriaceae group of bacteria. The test system consists of a 5.0 psid pressure transducer and a pressure equalizer valve attached to the metal cap of a 20 x 150 mm test tube with gas pressure being recorded on a strip-chart recorder.

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Biocontrol microorganisms

       Methods, devices, and compositions described herein are directed to artificially evolving an organism for use as a biocontrol agent. Methods, devices, and compositions described herein are useful for evolving a microorganism to acquire traits not naturally associated with the microorganism. The artificial evolution process can utilize culture methods and devices designed to accommodate particular culture methods described herein. The organism can be artificially evolved for a characteristic such as ultraviolet light tolerance, chemical tolerance, thermotolerance, enhanced growth rate on a target carbon source, host specific growth, modified sporulation characteristics or modified spores.

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Microorganism detecting system and microorganism detecting method

       The present invention relates to a microorganism detecting system comprising: a microorganism detecting device 20 for detecting fluorescent light produced by a microorganism included in a solution to detect a number of microorganisms included in the solution; a relationship storing device 351 for storing a correlation relationship between a number of microorganisms in the solution and a number of colonies formed by the microorganisms on a culture medium; and a converting portion 301 for using the correlation relationship to convert the number of microorganisms, detected by the microorganism detecting device 20, into a number of colonies that may be formed.

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Bacteriophages for use against bacterial infections

       The present invention relates to a composition comprising obligate lytic bacteriophages generated by a method comprising subjecting normally in vivo lysogenic, pseudolysogenic or temperate bacteriophages to genetic modifications in vitro, which alters the biological activity of one or more of the individual gene products for establishing, maintaining, controlling or regulating the lysogenic life cycle of the bacteriophages, thereby converting them to obligate lytic bacteriophages, wherein the genetic modification includes modification of a single gene in the operon containing a gene resulting in a gene product for establishing, maintaining, controlling or regulating the lysogenic life cycle of the bacteriophages.

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Methods of hydrolyzing pretreated densified biomass particulates and systems related thereto

       A method is provided in which pretreated and densified cellulosic biomass particulates can be hydrolyzed at a high solids loading rate as compared with the solids loading rate of loose hydrolysable cellulosic biomass fibers. The resulting high concentration sugar-containing stream can be easily converted to biofuels or an entire suite of other useful bioproducts.

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Method to increase the production of extracellular polymeric substances (EPS) in a Acidithiobacillus ferrooxidans culture by the inhibition of enzymes of tricarboxilic acid cycle

       A method of increasing the production of extracellular polymeric substances (EPS) in an Acidithiobacillus ferrooxidans culture is disclosed. The method includes inhibiting an enzyme, such as citrate synthase, aconitase, or isocitrate dehydrogenase, in the tricarboxylic acid (TCA) cycle leading to alpha-ketoglutarate.

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Compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa

       The present invention provides compositions, methods and kits for the species-specific detection of Pseudomonas aeruginosa.

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Microbial mediated chemical sequestering of phosphate in a closed-loop recirculating aquaculture system

       A system and method for removal of phosphates from a closed loop estuarine or marine recirculating aquaculture system (RAS), involving denitrification in the presence of a media substrate for: 1) anaerobic nitrate, nitrite and ammonia removal and 2) sequestering of excess phosphate in the RAS. Phosphate is precipitated in or on the denitrifying biofilm formed on the media substrate as phosphate salts. The phosphate salt-containing media substrate can be removed from the RAS, the phosphates can be removed, and both the substrate and the phosphates can be reused or recycled.

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Toxin-eating bacteria and bioremediation

       Methods are provided for reducing a level of one or more antibiotics from an antibiotic-contaminated substance. An organism that can utilize the one or more antibiotics as a carbon source is cultured in the presence of the antibiotic-contaminated substance for a sufficient amount of time to reduce the level of one or more antibiotics from the antibiotic-contaminated substance.

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Method of biological permeability modification of oil reservoirs using resident microorganisms and nutrient supplementation

       A method of increasing oil recovery from oil reservoirs includes the step of conducting a series of lab analysis that lead to a specific nutrient formulation followed by or in parallel with a genetic analysis of resident microorganisms in water produced from an oil reservoir to determine if the population of oil interactive microorganisms may be modified to produce a change in oil-water relative permeability of an oil reservoir. The series of analyses are conducted to determine the contents of a specific nutrient formulation package to optimize changes in the resident microorganisms to enhance oil recovery by changing oil-water relative permeability in the oil reservoir. Next the specific nutrient formulation is applied to stimulate some of the resident microorganisms to interact with oil contained in the reservoir rock formations. The stimulation step is followed by limiting the specific nutrient formulation to alter or modify the physiology and surface characteristics of the targeted microorganisms.

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Automated characterization and classification of microorganisms

       An instrument, method, use and software program to obtain information rapidly about microorganisms that may spread uncontrolled in hospitals, water supply, food or when used in bio terrorism are described. Vibrational spectroscopy provides data to a computer linked to one or more databases. Comparison of the spectral data and information retrieved from the databases is used to identify and classify the microorganisms, applying suitable algorithms, which algorithms are self-generating and self-adapting to new spectroscopic data. The system may alert for the detection of an outbreak or to take disinfection measures. Changes in the traditional taxonomic division of microorganisms have no influence on the instrument. It does not rely on an a priori knowledge about the taxonomic classification of the microbial strain, is straightforward and easily integrated in routine microbial procedures.

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Compositions for inactivating pathogenic microorganisms, methods of making the compositions, and methods of use thereof

       Nanoemulsion compositions with low toxicity that demonstrate broad spectrum inactivation of microorganisms or prevention of diseases are described. The nanoemulsions contain an aqueous phase, an oil phase comprising an oil and an organic solvent, and one or more surfactants. Methods of making nanoemulsions and inactivating pathogenic microorganisms are also provided.

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Methods for increasing the production of ethanol from microbial fermentation

       A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.

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Process for production of alcohols by microbial fermentation

       The invention provides method for producing butanediol and specifically 2,3-butanediol by anaerobic fermentation of a gaseous substrate comprising CO by a culture of at least one micro-organism. In accordance with particular methods of the invention, 2,3-butanediol is produced by anaerobic fermentation of substrates including carbohydrates and carbon monoxide. The invention further provides for the up-regulation of a native 2,3-butanediol dehydrogenase gene in the at least one micro-organism. The 2,3-butanediol can be further converted to compounds such as butene(s), butadiene and methyl ethyl ketone. These compounds can be further converted to chemical products such as 2-butanol.

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System and method for controlling metabolite production in a microbial fermentation

       A method is provided for controlling a metabolic profile of an anaerobic microbial fermentation culture. In particular, a metabolic profile of a fermentation process is controlled by controlling the amount of dissolved CO2 provided to a culture. Further provided is a method of producing one or more products by microbial fermentation of a gaseous substrate through feeding tail gas CO2 from a reactor to a second reactor, or by recycling tail gas CO2 to the same reactor.

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Ammonia-specific 5'-XMP aminase mutant

       Disclosed herein are ammonia-specific 5'-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5'-XMP aminase. The resulting 5'-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5'-XMP into 5'-GMP. Thus, the ammonia-specific 5'-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5'-XMP into 5'-GMP.

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"Antimicrobial compounds having protective or therapeutic leaving groups, Paul Hsien-Fu Wu, Catherine E. Taylor, Linnus Cheruiyot, Jianwei Li, Terese A. Bartlett, Matt Bergan, Less, Patent number: US8679518 B2, 2014.


Method and apparatus for detecting degree of "spoilage of food", Alfano, Robert R, Pu, Yang, Wang, Wubao, Patent number: US 20140300891 A1, 2014.


"Antimicrobial compounds", Werner Hölzl, Andrea Preuss, Michèle Gerster, Patent number US8637694 B2, 2014.


"Microorganisms for therapy", Aladar A. Szalay, Tatyana Timiryasova, Yong A. Yu, Qian Zhang, Patent number US 8784836 B2, 2014.


"Vaccinia virus for diagnosis and therapy of tumors", Aladar A. Szalay, Yong A. Yu, Shahrokh Shabahang, Tatyana Timiryasova, Patent number US8642257 B2, 2014.


"Patent Picks—Selective Microbial Growth/Inhibition, Gentle Disinfectants, Improved Resistance and More", Rachel L. Grabenhofer, 2013.


"Bioplastic molded body and method for producing bioplastic molded body", Kiuchi Yukihiro, Patent number WO 2013047285 A1, 2013.


"Biocontrol of nematodes", Reed Nathan Royalty, Varghese THOMAS, Roy WHITSON, Patent number WO 2013016328 A1, 2013.


"Compositions and methods to prevent and treat biofilms", Svetlana A. IVANOVA, Dennis W. Davis, Brad W. ARENZ, Thomas K. CONNELLAN, Patent number WO 2012173796 A3, 2013.


"Processs of electronic management of organic production and certification", Sitaram Prasad Paikray, C.R Hazra, P Das, Patent number US20130041833 A1, 2013.


"Methods for the economical production of biofuel from biomass", Andrew C. Hawkins, David A. Glassner, Thomas Buelter, James Wade, Peter Meinhold, Matthew W. Peters, Patrick R. Gruber, William A. Evanko, Aristos A. Aristidou, Marco Landwehr, Patent number US8431374 B2, 2013.


"Biocontrol microorganisms", Crecy Eudes De, Patent number EP2467023 A2, 2012.


"Genetically modified plants which synthesize a low amylose starch with increased swelling power", Claus Frohberg, Ralf-Christian Schmidt, Patent number US8252975 B2, 2012.


"Plant growth regulation", Ulrich Johannes Haas, Tyler L. Harp, Patent number EP2503884 A1, 2012.


"Testing of Biofilm for Anti-microbial Agent Susceptibility", Merle E. Olson, Howard Ceri, Patent number US20120329675 A1, 2012.


"Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids", Aristos Aristidou, Frances Arnold, Sabine Bastian, Ruth Berry, Thomas Buelter, Melissa Dey, Catherine Asleson Dundon, Kent Evans, Andrew Hawkins, Justas Jancauskas, Julie Kelly, Doug Lies, Peter Meinhold, Matthew Peters, Stephanie Porter-Scheinman, Jun Urano, Patent number WO2011142865 A3, 2012.


"Pseudozyma aphidis as a biocontrol agent against various plant pathogens", Aviva Gafni, Marganit Levy, Patent number WO 2011151819 A3, 2012.


"Method for controlling microbial biofilm in aqueous systems", Paul Frail, Patent number WO2012044409 A1, 2012.


"A plant growth promoting composition", Swee Chine Jenny Lee, Tik Voon Liew, Patent number WO 2012081970 A3, 2012.


"Soil activating powder",Hua Juan, SUN Hailong, Shao before, Yang Tao, China Juan - Ecological Technology Co., Ltd. Sichuan Lizi, Patent number CN 101747903 B, 2011.


"Materials and methods for assessing and mapping microbes and microbial biofilms on wounds", Gregory S. Schultz, Priscilla Lorraine Phillips, Edith Marion Sampson, Patent number WO 2010124277 A3, 2011.


"Rapid detection method for microorganisms using nanoparticles", Min Gon Kim, Yong Beom Shin, Yoon Joo Sung, Patent number WO2011065747 A3, 2011.


"Carbon-negative bio-plastic furniture", Stuart B. Smith, Patent number WO2011017271 A8, 2011.


"Flexible biofuel cell, device and method", Tautgirdas Ruzgas, Sergey Shleev, Thomas Arnebrant, Patent number WO2011117357 A3, 2011.


"Method for controlling microbial biofilm in aqueous systems", Gloria Jean Tafel, Wilson Kurt Whitekettle, Qing Zhao, Patent number EP2173670 A1, 2010.


"A method of extracting and purifying polyhydroxy alkanoate bioplastic", Mohd. Aii Hassan, Patent number EP 2220142 A1, 2010.


"Assay for detecting nucleotide sequences in genetically modified crops and plants using optical thin-film biosensor chips", Xing Wang Deng, Patent number US20090118136 A1, 2009.


"Method for controlling microbial biofilm in aqueous systems", Gloria Jean Tafel, Wilson Kurt Whitekettle, Qing Zhao, Patent number WO2009020694 A1, 2009.


"Composition for increasing soil fertility", Larry Lancaster, Patent number WO 2008033443 A3, 2008.


"Solid product effective for biocontrol of vascular fusariosis of melon, method for preparation thereof and method for use of same", Garcia Ana Isabel Anton, Vicente Agustina Bernal, Izquierdo Carlos Javier Garcia, Fernandez Maria Teresa Hernandez, Mondejar Ruben Lopez, Medina Ainhoa Martinez, Villamor Noelia Navarro, Patent number WO 2008077982 A1, 2008.


"An assay for detecting nucleotide sequences in genetically modified crops and plants using optical thin-film biosensor chips", Xing Wang Deng, Patent number WO2008085540 A3, 2008.


"Aquacultural farming method with recycling of organic and mineral elements", Christian Drakides, Sylvain Gilles, Patent number EP1729568 B1, 2008.


"Methods for microbial biofilm destruction and interference with microbial cellular physiology", Renee M. Saville, Alfred M. Spormann, Kai M. Thormann, Patent number WO2006045041 A3, 2007.


"Preparation for regulating the pH factor of the soil in combination with an improvement of soil fertility", Iradj Scharafat, Patent number EP1712535 A1, 2006.


"Bioplastic and preparation method thereof", Montes Crispulo Gallegos, Patent number WO2006134188 A2, 2006.


"Post harvest control of genetically modified crop growth employing d-amino acid compounds", Marcus Ebneth, Patent number WO2005090582 A1, 2005.

 
 
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