Byung-Yong Kim, Xiaoying Rong, Tiago D. Zucchi, Avinash N. V. Bonda, Ying Huang and Michael Goodfellow
School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
The taxonomic positions of three streptomycetes isolated from a soil sample from a hay meadow were determined using a polyphasic approach. The isolates had chemical and morphological properties typical of the genus Streptomyces and, in phylogenetic analyses based on 16S rRNA gene sequences, formed a distinct subclade that was most closely related to the Streptomyces prasinus subclade. DNA–DNA relatedness studies showed that the novel strains belonged to three different genomic species. The novel strains could be distinguished from one another and from the type strains of the species classified in the S. prasinus subclade using a combination of genotypic and phenotypic properties. On the basis of these data, it is proposed that the novel strains be assigned to the genus Streptomyces as Streptomyces herbaceus sp. nov., Streptomyces incanus sp. nov. and Streptomyces pratens sp. nov., with BK119T (=KACC 21001T=CGMCC 4.5797T), BK128T (=KACC 21002T=CGMCC 4.5799T) and BK138T (=KACC 20904T=CGMCC 4.5800T) as the respective type strains.