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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)
Vol. 250, 20
14; Page: 1023 - 1032

Isolation and Characterization of a New Moderately Halophilic Bacterium Strain SM. 200-5 from Solar Saltern Ponds

Gaochao Xu, Yuangao Deng, Donghui Song, Liying Sui

Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China.

Abstract

A new moderately halophilic bacterium strain SM. 200-5 was isolated from the solar saltern ponds with salinity 200 in Hangu Saltworks, Tianjin, China. The cells of the strain SM. 200-5 were rods and Gram-negative. They could grow in a salinity range of 30–150 and initial pH 6–11, with optimum of salinity 100 and initial pH 7. 16S rDNA alignment showed that the strain SM. 200-5 had 93–95 % similarity with those sequences of genus Salimicrobium sp.. Biochemical characterization analysis via API 20E system indicated that biochemical characters of the strain SM. 200-5 were partially different from Salimicrobium sp. ISL-25, which was its closest member in the phylogenetic tree. Therefore, the strain SM. 200-5 proposed a new species of Salimicrobium.

Keywords: Halophilic bacterium; Phylogenetic analysis; Salimicrobium sp.; 16S rDNA


 

 

 
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