Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Research on Microbes
Microbiology Experts
Online Submission
Access Statistics

Site Visitors

blog tracking

Vol: , No: , 2006; Pages:

Inference of bacterial microevolution using multilocus
sequence data

Xavier Didelot and Daniel Falush

Peter Medawar Building for Pathogen Research, South Parks Road. Oxford OX1 3SY, UK.


We describe a model-based method for using multilocus sequence data to infer the clonal relationships of bacteria and the chromosomal position of homologous recombination events that disrupt a clonal pattern of inheritance. The key assumption of our model is that recombination events introduce a constant rate of substitutions to a contiguous region of sequence. The method is applicable both to Multilocus Sequence Typing (MLST) data from a few loci and alignments of multiple bacterial genomes. It can be used to decide whether a subset of isolates share common ancestry, to estimate the age of the common ancestor and hence to address a variety of epidemiological and ecological questions that hinge on the pattern of bacterial spread. It should also be useful in associating particular genetic events with the changes in phenotype that they cause. We show that the model outperforms existing methods of subdividing recombinogenic bacteria using MLST data and provide examples from Salmonella and Bacillus.

Keywords:Multilocus sequence typing, BURST, genealogical inference, comparative genomics, recombination.

Corresponding author: Tel 44 01865 281546 ,Fax +44 1865 272595.

E-mail: falush@stats.ox.ac.uk


Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution