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Med Dosw Mikrobiol
Vol. 63, No: 2, 2011, Pages: 131 - 8(Polish)

Evaluation of biofilm formation by Proteus mirabilis strains on the surface of different biomaterials by two methods

Kwiecinska-Piróg J, Bogiel T, Gospodarek E

Katedra i Zaklad Mikrobiologii, Collegium Medicum im. L. Rydygiera w Bydgoszczy, Uniwersytet Mikolaja Kopernika w Toruniu.


Proteus sp. rods are opportunistic human pathogens. These microorganisms are mainly isolated from patients with urinary tract infections, particularly associated with using of biomaterials, on which surface they can form biofilm. The aim of our study was the estimation of Proteus mirabilis rods ability to form biofilm on the surface of 5 biomaterials (polychloride vinyl, silicone latex, polypropylene, polybutylen teraftalan and polyamide) using Richards' and quantitative method and comparison results of both methods. A total number of 84 P. mirabilis strains were included into the study. All of them were isolated in the Department of Clinical Microbiology University Hospital no. 1 of dr A. Jurasz Collegium Medicum of L. Rydygier in Bydgoszcz, Nicolaus Copernicus University in Toruń between 2005 and 2008. Examined P. mirabilis strains formed heavy biofilm with statistically significantly values on the surface of silicone latex than on polychloride vinyl and on polypropylene surface than polybutylen teraftalen or polyamide. High correlation of both methods was established. The Richards' method can be used to quick identification of P. mirabilis biofilm.

Keywords:polychloride vinyl, silicone latex, polypropylene, polybutylen teraftalan and polyamide,human pathogens.


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