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Archives of Oral Biology
Vol. 81, 2017, Pages: 97-102

Putative periodontal pathogens in the subgingival plaque of Sudanese subjects with aggressive periodontitis

N.T.Hashim, G.J.Linden, L.Winning,M.E.Ibrahim, B.G.Gismalla, F.T.Lundy, I.A.El Karim

Faculty of Dentistry, University of Khartoum, Khartoum, Sudan.

Abstract

Background and objectives

There has been limited study of the bacterial species associated with aggressive periodontitis (AgP) in high-risk populations in Africa. The aim of this study was to investigate and quantify the presence of four putative periodontal pathogens in the subgingival plaque of Sudanese subjects with AgP. A secondary aim was to investigate the effect of varying the detection threshold on the reported prevalence of the bacterial species investigated.

Materials and methods

Subgingival plaque samples were collected from AgP cases (n = 73) and healthy controls (n = 71). Bacterial DNA was extracted and analyzed by quantitative polymerase chain reaction for the detection and quantification of four putative periodontal pathogens: Porphyromonas gingivalisAggregatibacter actinomycetemcomitansTreponema denticolaand Tannerella forsythia.

Results

At the lowest detection threshold (>101 cells), P. gingivalis (p < 0.0001) was more prevalent in AgP cases than controls. T. forsythia and T. denticola had a high prevalence (>70%) in AgP cases at all detection levels. While T. forsythia was significantly more frequently identified in AgP than in controls at all detection thresholds, this was only the case for T. denticola at the intermediate threshold (>102 cells). A. actinomycetemcomitans was identified less frequently than the other bacterial species with no difference in its prevalence between AgP cases and controls.

Conclusion

The prevalence of the putative periodontal pathogens investigated varied considerably in Sudanese subjects with AgP and in periodontally healthy controls depending on the detection thresholds applied. T. forsythia was identified as having the strongest association with AgP.

Keywords: Aggressive periodontitis, Periodontal pathogens.
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