Precision Breeding Made Real with CRISPR: Illustration through Genetic Resistance to Pathogens
Florian Veillet1,2,4, Mickael Durand3, Thomas Kroj4, Stella Cesari4, Jean-Luc Gallois5
IGEPP, INRAE, Institut Agro, Univ Rennes, Ploudaniel 29260, France.
Since its discovery as a bacterial adaptive immune system and its development for genome editing in eukaryotes, the CRISPR technology has revolutionized plant research and precision crop breeding. The CRISPR toolbox holds great promise in the production of crops with genetic disease resistance to increase agriculture resilience and reduce chemical crop protection with a strong impact on the environment and public health. In this review, we provide an extensive overview on recent breakthroughs in CRISPR technology, including the newly developed prime editing system that allows precision gene editing in plants. We present how each CRISPR tool can be selected for optimal use in accordance with its specific strengths and limitations, and illustrate how the CRISPR toolbox can foster the development of genetically pathogen-resistant crops for sustainable agriculture.
Keywords : CRISPR-Cas9, gene targeting, base editing, prime editing, plant/pathogen interactions, precision crop breeding.