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Sensors and Actuators B: Chemical
Volume 325, 2020, 128748

Nanoporous layer fiber biosensing platform for real time culture- and separation-free detecting bacterial pathogens and measuring their susceptibility to antibiotics

Shunyan Fanga, Dan Songa, Anna Zhub, Feng Longa

School of Environment and Natural Resources, Renmin University of China, Beijing, 100874, China.

Abstract

Simple, rapid, and sensitive detection of bacterial pathogens in environmental and clinical samples is a key requirement because of the ensuing high morbidity and mortality. In this study, a nanoporous layer fiber biosensing platform (called NFB) was developed for the rapid culture- and separation-free detection of bacterial pathogens through integrating evanescent wave fluorescence technology, the size effect of nanoporous layer, and real-time aptasensors. The in-situ etched nanoporous layer of the fiber probe can not only effectively distinguish free Cy5.5-aptasensors and Cy5.5-aptasensors bound to pathogens but also enhance evanescent wave fluorescence. NFB can selectively and sensitively quantify Escherichia coli O157:H7 and Salmonella typhimurium species in environmental and blood samples within 18 min and 32 min, respectively. NFB detected a significant increase in fluorescence when the mixture of pathogens and Cy5.5-aptasensors was exposed to antibiotics. Our results clearly demonstrated that the portable NFB can serve as a powerful tool for real time detection of pathogens and measurement of their response to antibiotics. .

Keywords: Nanopore, Bacterial pathogen, Aptamer, Fiber optic biosensor, Evanescent wave.

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