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Mycologia
Vol. 97, No. 3, 2005; Pages: 612–620


Molecular and genetic analyses of geographic variation in isolates of Phoma macrostoma used for biological weed control

Lecong Zhou K.L. Bailey1 C.Y. Chen Mario Keri

Plant Pathology and Biocontrol Unit, SLU, Box 7035, 75007 Uppsala, Sweden.

Abstract

Molecular and genetic approaches were used to evaluate the genetic relatedness among isolates of the fungus Phoma macrostoma Montagne originating from Canada and Europe and to other species in the genus Phoma. Distinct differences were observed in genetic variation among nine species of the genus Phoma. Randomly amplified polymorphic DNA (RAPD) revealed the presence of intraspecific genetic variation among the isolates of P. macrostoma, with the isolates being used for biological weed control being distributed in a distinct phylogenetic cluster. Additional variation within the biocontrol isolate cluster in P. macrostoma was revealed by pulsed field gel electrophoresis (PFGE), which showed that biocontrol isolates generated two different chromosomal profiles, however the profiles did not relate to their Canadian ecozone origin. Mating studies showed that biocontrol isolates of P. macrostoma from Canada did not produce sexual reproductive structures and were incapable of crossing. These studies also confirmed that no obvious differentiation exists among the biocontrol isolates of P. macrostoma from Canadian Ecozones 3 and 4.

Keywords:biocontrol, genetic variation, mating, PFGE, Phoma macrostoma, RAPD,Randomly amplified polymorphic,microorganisms,Leptosphaeria maculans,pathogens.


Corresponding author: Tel +1 (306) 956 7260; Fax +1 (306) 956 7247

E-mail: BaileyK@agr.gc.ca

Department of Zoology, Life Science Building, University of Madras, Guindy Campus, Chennai - 600 025. Tamil Nadu, India. Phone:91-44-22300899, Fax : 044-22300899; Email: dzum@envis.nic.in; enviscoordinator@gmail.com
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