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International Journal of Food Microbiology
164, No. 1, 2013; Pages: 70 - 75

In-situ immuno-gold nanoparticle network ELISA biosensors for pathogen detection

Il-Hoon Cho, Joseph Irudayaraj

Bindley Bioscience and Birck Nanotechnology Center, Department of Agricultural & Biological Engineering, Purdue University, West Lafayette, IN 47907, United States


Food poisoning microorganisms that contaminate food products and compromise food safety and security have been considered a major health threat and a serious concern for food producers and processors. Developing sensor technologies that are rapid for sensitive and selective detection and quantification of pathogens is a high priority for scientists in academia, state and federal research institutes, and industries. In this work we propose an in-situ immuno-AuNP network-based ELISA biosensor integrated with a sample concentration step based on immuno-magnetic separation to detect pathogenic microorganisms with high sensitivity. The sensor system was optimized by the specific formation of immuno-AuNP network onto the antigenic site present at the outer membrane surface of bacteria and the analytical concept was validated by a microtiter immunoassay. The in-situ network biosensor was able to detect pathogens at extremely low numbers: 3 cells/mL of Escherichia coli O157:H7 and Salmonella typhimurium in buffer and 3 CFU/mL of E. coli O157:H7 and 15 CFU/mL of S. typhimurium in real sample conditions within 2 h of inoculation. The ability to monitor target bacteria with improved analytical sensitivity compared to the current techniques presents a unique opportunity for routine monitoring to improve the safety of foods.

Keywords: Foodborne pathogens; Rapid detection; Sensitive; Immuno-magnetic separation; Immuno-gold nanoparticles



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