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Aquaculture Reports
Volume 21, 2021, 100782

Galectin-related protein from Oreochromis niloticus: Molecular, and transcriptional features and biological responses against microbial pathogens

Guoling Luoa,b,c,1, Jinzhong Niua,b,c,1, Junfeng Fanga,1, Jufen Tanga,b,c,1, Yishan Lua,b,c,1, Jichang Jiana,b,c,1

Fisheries College of Guangdong Ocean University, Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China.

Abstract

Galectin is a kind of lectin that can promote the cell adhesion, proliferation, and phagocytosis and participates in immune regulation and destruction of microbes. In this study, a galectin-related protein (On-GRP) homolog was identified from Nile tilapia, and its expression level and antibacterial ability were studied. The Open Reading Frame, which encoded a 179 amino acid protein (GenBank accession no: XM_005733705.1), was 537 bp. The On-GRP possessed a carbohydrate recognition domain. qRT-PCR showed that the On-GRP was detected in the examined tissues in healthy Nile tilapia, and the highest expression levels was observed in the skin. After challenge with Streptococcus agalactiae, the On-GRP expression was up-regulated significantly (P < 0.05) in the whole blood, head kidney, gills and thymus. In addition, the recombinant On-GRP (rOn-GRP) could agglutinate and bind Streptococcus agalactiae and Aeromonas hydrophila. In vitro and in vivo bacteriostatic tests showed that the rOn-GRP could inhibit the growth of bacteria. Besides, the rOn-GRP could promote the phagocytic ability of macrophages to engulf bacteria. Over all, this study indicated On-GRP is involved in the immune responses of Nile tilapia against bacterial pathogens.

Keywords Nile tilapia, Galectin-related protein, Bacterial infection, Immune response.

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