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Human Fungal Pathogen Identification
Vol. 1508, 2016, Pages: 141-150

Fungal-Grade Reagents and Materials for Molecular Analysis

Michael G. Lorenz, Michael Lustig, Marina Linow

Molzym GmbH & Co. KG, Mary-Astell-Str. 10, Bremen, 28359, Germany.


Fungal DNA is present at very low loads in clinical specimens. Molecular detection by amplification assays generally is a challenge because of a potentially multiple input of contaminating DNA from exogenous sources. Besides airborne, handling and cross-contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution decontamination procedures of materials and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction, and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the diagnosis of fungal organisms at the limit of detection.

Keywords: Fungal DNA, Contamination, Decontamination, DNA-free, Fungal PCR reagents, Fungal DNA extraction, Fungal assays.

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