3 7
Home About us MoEF Contact us Sitemap Tamil Website  
About Envis
Whats New
Microorganisms
Research on Microbes
Database
Bibliography
Publications
Library
E-Resources
Microbiology Experts
Events
Online Submission
Access Statistics

Site Visitors

blog tracking


 
Human Fungal Pathogen Identification
Vol. 1508, 2016, Pages: 141-150

Fungal-Grade Reagents and Materials for Molecular Analysis

Michael G. Lorenz, Michael Lustig, Marina Linow

Molzym GmbH & Co. KG, Mary-Astell-Str. 10, Bremen, 28359, Germany.

Abstract

Fungal DNA is present at very low loads in clinical specimens. Molecular detection by amplification assays generally is a challenge because of a potentially multiple input of contaminating DNA from exogenous sources. Besides airborne, handling and cross-contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution decontamination procedures of materials and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction, and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the diagnosis of fungal organisms at the limit of detection.

Keywords: Fungal DNA, Contamination, Decontamination, DNA-free, Fungal PCR reagents, Fungal DNA extraction, Fungal assays.

Copyright © 2005 ENVIS Centre ! All rights reserved
This site is optimized for 1024 x 768 screen resolution