APPLIED
AND ENVIRONMENTAL MICROBIOLOGY
Vol. 74, No.10 , 2008; Pages:3295–3301
Assessment
of the Genetic Diversity of Xanthomonas axonopodis
pv. phaseoli and Xanthomonas fuscans subsp.
fuscans as a Basis To Identify Putative Pathogenicity
Genes and a Type III Secretion System of the SPI-1 Family
by Multiple Suppression Subtractive Hybridizations
Seyed Mehdi Alavi,1 Saeideh Sanjari,1
Fabien Durand,1 Chrystelle Brin,1
Charles Manceau,1†* and Stephane
Poussier2†*
UMR PaVe, INH, 2 rue Le Notre, 49045 Angers Cedex
1, France.
Abstract
Fluorescent amplified fragment length
polymorphism revealed that strains of Xanthomonas
axonopodis pv.
phaseoli and Xanthomonas fuscans subsp. fuscans
are genetically distinct and can be grouped into four
genetic
lineages. Four suppression subtractive hybridizations
were then performed to isolate DNA fragments present
in these bean pathogens and absent from closely related
xanthomonads. Virulence gene candidates were
identified such as homologs of hemagglutinins, TonB-dependent
receptors, zinc-dependent metalloproteases,
type III effectors, and type IV secretion system components.
Unexpectedly, homologs of the type III secretion
apparatus components (SPI-1 family), usually reported
in animal pathogens and insect symbionts, were also
detected.
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