Purpose: Urinary tract infections are a serious health problem affecting millions of people each year. Therefore, studies for new alternative remedies are necessary. The aim of this study was to investigate the antimicrobial potential of the leaves of Stachys pseudopinardii R. Bhattarcharjee & Hub.-Mor. (Lamiaceae) against the pathogens causing complicated urine tract infections. Methods: The ethanolic extracts obtained from the leaves of S. pseudopinardii were investigated for their antimicrobial activities against the pathogens causing complicated urine tract infections (Enterococcus feacalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis and Candida albicans) by disc diffusion method and microdilution method. Some antibacterial and antifungal antibiotics were used as a positive reference standard to determine the sensitivity of the strains. Results: The extracts showed antimicrobial activity against Enterococcus faecalis, Proteus mirabilis and Candida albicans with inhibition zones of 18.2, 17.4 and 16.0 mm, with MIC's and MBC's or MFC's of 32.0 (64.0), 32.0 (64.0) and 64.0 (128.0) µg/mL, respectively. Also the extracts exhibited moderate activity against the other test microorganisms. Conclusion: Our findings support the use of S. pseudopinardii in traditional medicine for the treatment against the urine tract pathogens. Hence, it is suggested to isolate and identify the active compounds of the plant for novel antimicrobial agents in future.