Antimicrobial activity of Azadirachta indica (Neem) against Pathogenic Microorganisms
Ranjit R. Raut, Ajit R. Sawant and Bhagyashree B. Jamge
Dept. of Biotechnology, Padmashri Vikhe Patil College of Arts, Science and Commerce, Pravranagar, Loni.Tal-Rahata,
Plants have been a source of herbal remedies throughout the history of mankind. Various medicinal plants
have been used for years in daily life to treat diseases all over the world. In the present study, antimicrobial
activity of Azadirachta indica was evaluated against gram negative pathogenic bacteria (Escherichia coli,
Salmonella typhi and Vibrio cholerae) and gram positive bacteria (Bacillus subtilis). Azadirachta indica leaves
and barks were collected from the fields of Botanical Garden in Padmashri Vikhe Patil College, Pravaranagar
and pure cultures of the test organisms used for antimicrobial study were obtained from the Dept. of
Microbiology, Pravara Institute of Medical Sciences, Loni. All the test organisms were screened for their
antibacterial activity against leaf and bark extract of A. indica by agar well diffusion method. Leaf and bark
extract of Azadirachta indica showed more inhibition zone against Vibrio cholerae and Bacillus subtilis, while
E. coli and S. typhi are less susceptible to neem extract.
Antimicrobial activity, Azadirachta indica, pathogenic bacteria, leaf and bark extract.