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Journal of Microbiological Methods
Vol. 127, 2016, Pages: 59–61

A rapid, highly sensitive and culture-free detection of pathogens from whole blood by removal of white blood cells using immuno-magnetic beads

Manjula Ramya Vutukuru, Divya Khandige Sharma, Ragavendar MS, Nivedita Mitra

Siemens Healthcare Private Limited, 105, Prestige Alecto, Hosur Road, Bengaluru 560100, India.

Abstract

Using anti-human CD45 antibody coated beads, we show a 98% reduction of WBCs from spiked blood samples in 1 h, thereby enriching it for pathogens. This enrichment allowed the detection of < 10 CFU of Escherichiacoli in 1 mL blood using quantitative PCR; something not observed in unenriched samples.

Graphical abstract

Keywords: Pathogen enrichment; Analytical sensitivity; Low pathogen load; Sepsis; Healthcare acquired infections; Blood stream infections; Culture-free detection; Quantitative PCR (qPCR); CD45; Molecular diagnostics and culture-free detection of pathogens from whole blood.

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