Rapid quantitative analysis of multi-components in Andrographis paniculata using UPLC-QqQ_LIT-MS/MS: Application to soil sodicity and organic farming

Preeti Chandra, Rekha Kannujia, Renu Pandey, Shipra Shukla, Lal Bahadur, Mahesh Pal, Brijesh Kumar

Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India.

Abstract

An ultra performance liquid chromatography-triple quadrupole-linear ion trap mass spectrometry method in multiple-reaction monitoring mode was developed for the rapid determination of 12 bioactive compounds in the leaf and stem of Andrographis paniculata(A. paniculata). Different soil conditions i.e., soil sodicity and use of organic manure was manually prepared and its effect on the extractive yield and quantity of bioactive phytoconstituents was studied. A good linear regression relationship (r², 0.9988–0.9999), intra-day precision (RSD, 0.17–3.22%), inter-day precision (RSD, 0.31–3.44%), stability (RSD, 1.19–3.09%), and recovery (RSD, 1.03–3.20%) were obtained for all the analytes. The results showed significant differences in the content of diterpenoids, flavonoids, phenolics and triterpenoid compounds. Major markers i.e., andrographolide and neoandrographolide (187.5 mg/g and 109.0 mg/g, respectively) were detected highest in silty clay loam soil with addition of 3.5% organic manure in leaf of A. paniculata. This study was designed to benefit the cultivators by suggesting them about the suitability of soil and manure conditions to enhance the quantity of major bioactive compounds from A. paniculata. The present work described a highly specific, sensitive and rapid determination method of 12 bioactive compounds.

Graphical abstract

Keywords: Quantification; UPLC-QqQ_LIT-MS/MS; Andrographis paniculata; Soil evaluation; Diterpenoids.