Prevalence and antimicrobial profile of Campylobacter isolates from free-range and conventional farming chicken meat during a 6-year survey

Vangelis Economou, Nikolaos Zisides, Panagiota Gousia, Stefanos Petsios, Hercules Sakkas, Nikolaos Soultos, Chrissanthy Papadopoulou

Food-Water Microbiology Group, Department of Microbiology, Faculty of Medicine, School of Health Sciences, University of Ioannina, Ioannina 45110, Greece.

Abstract

The present study aimed to investigate the prevalence of Campylobacter spp. in the meat of free-range and conventional farming broilers and to assess the respective antimicrobial susceptibility of the isolates. Three hundred and sixty nine fresh chicken meat samples were collected from different farms at slaughter (60 from free-range poultry farms and 309 from conventional farms) in North Western Greece. Susceptibility tests against 12 antimicrobials (amikacin, gentamicin, imipenem, cefamandole, cefotaxime, cefoxitin, imipenem, erythromycin, ampicillin, amoxicillin – clavulanic acid, ciprofloxacin, tetracycline) were performed. Campylobacter spp. was isolated from 91 (29.4%) of the free-range chicken meat and from 106 (28.7%) of the conventional farming chicken meat samples. The annual incidence of Campylobacter spp. among the free-range and conventional broilers was not statistically significant. However, a reducing trend was observed in the overall prevalence of Campylobacter spp. from 2005 to 2010. Variable resistance rates were observed towards the tested antimicrobials: erythromycin (76%), tetracyclines (71%), ampicillin (66%), ciprofloxacin (51%), cefamendole (41%) cefoxitin (27%), amikacin (15%), and cefotaxime (5%). No differences were observed in the antimicrobial susceptibility of isolates from free-range and conventional farming samples.

Keywords: Campylobacter; Susceptibility; Chicken; Broilers; Free-range; Conventional farming.