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Int.J.Curr.Microbiol.App.Sci
Vol. 5 (3), 2016, Pages: 745-763

Screening, Identification, Characterization and Production of Bacterial Lipase from Oil Spilled So

Mahima Golani, Krishnan Hajela and G. P. Pandey

School of Life Sciences, Devi Ahilya University, Indore (M.P.), India.

Abstract

A bacterial strain isolated from oil spilled soil and identified as Staphylococcus chromogenes O1A by morphological, cultural, biochemical tests and 16S rDNA gene sequence analysis with maximum lipase activity (3.1) was screened on tributyrin medium. Maximum lipase production was observed at 48 h of growth (2.30 µM/min/mg), 37°C temperature (2.30 µM/min/mg) and pH 7.0 (7.96 µM/min/mg) with agitation of 130 rpm (3.02 µM/min/mg). Yeast extract (2.07 µM/min/mg) was found to be the best nitrogen source (1%). Lactose was used as a non Lipidic carbon source (1%) for optimum production of lipase (1.69 µM/min/mg). Of the natural oils, Sunflower oil was able to induce more lipase (3.87 µM/min/mg). Among the metal ions tested Ca2+ enhanced the lipase activity (1.67 µM/min/mg) and Zn2+ showed lower activity (0.28 µM/min/mg).The enzyme was found to be stable in 0.5 % solutions of different Surfactants such as SDS, Triton X-100 & Tween80. On the basis of optimized parameters the lipase activity in newly designed production medium 3 was found to be higher (18.18 µM/min/mg).

Keywords: Lipase, Screening, Staphylococcus chromogenes O1A, Characterization, Production, Oil spilled soil.

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